Journal: Cell Reports

Article Title: Structural conservation of Lassa virus glycoproteins and recognition by neutralizing antibodies

doi: 10.1016/j.celrep.2023.112524

Figure Lengend Snippet:

Article Snippet: APC-Cy7 Mouse Anti-Human CD3 , BD Biosciences , Cat# 557757; RRID: AB_396863.

Techniques: Recombinant, Electron Microscopy, Mass Spectrometry, Sequencing, Lysis, Transfection, Luciferase, Plasmid Preparation, Software, Single-cell Analysis, Mutagenesis, Ligation, Nano Differential Scanning Fluorimetry, Expressing, Staining, Amplification, Inverted Microscopy, Microscopy, Microarray

Journal: STAR Protocols

Article Title: Chimerism and phenotypic analysis of intraepithelial and lamina propria T cells isolated from human ileal biopsies after intestinal transplantation

doi: 10.1016/j.xpro.2023.102192

Figure Lengend Snippet: Primary surface antibody staining panel: Pt25 multilineage panel

Article Snippet: Mouse monoclonal anti-Human CD3 (clone SK7) APC-Cy7 , BD Biosciences , Cat#561800; RRID: AB_396890.

Techniques: Staining

Journal: STAR Protocols

Article Title: Chimerism and phenotypic analysis of intraepithelial and lamina propria T cells isolated from human ileal biopsies after intestinal transplantation

doi: 10.1016/j.xpro.2023.102192

Figure Lengend Snippet: Gating strategy for multilineage chimerism, as well as donor and recipient T cell phenotypes, in post-transplant ileal biopsies, using ITx Pt25 POD241 IEL as an example (A) Representative contour plots depicting gating strategy of HLA-ABC + CD45 + cells, CD3 + T cells, CD3 + γδTCR + T cells, CD3 + γδTCR − CD4 + T cells, CD3 + γδTCR − CD8 + T cells, CD19 + B cells, and CD3 - CD56 + NK cells. (B) Percentages of donor (“D”) and recipient (“R”) multilineage chimerism in Pt25 POD241 IELs. Pt25’s donor is HLA-B12 + . Pt25, the recipient, is HLA-A68 + , which has serological cross-reactivity with HLA-A28. (C) Gating strategy for donor (“D”) and recipient (“R”) CD4 + and CD8 + T cells in Pt25 POD241 IELs. CD45RA vs. CCR7 to categorize naïve T cells (CD45RA + CCR7 + ), central memory T cells (TCMs: CD45RA − CCR7 + ), effector memory T cells (TEMs: CD45RA − CCR7 − ), and terminally-differentiated effector memory T cells (TEMRAs: CD45RA + CCR7 - ), tissue-resident memory T cells (TRMs: CD69 + CD103 +/− CD49a +/ − ), effector T cells (CD28 + NKG2D +/− ).

Article Snippet: Mouse monoclonal anti-Human CD3 (clone SK7) APC-Cy7 , BD Biosciences , Cat#561800; RRID: AB_396890.

Techniques:

Journal: STAR Protocols

Article Title: Chimerism and phenotypic analysis of intraepithelial and lamina propria T cells isolated from human ileal biopsies after intestinal transplantation

doi: 10.1016/j.xpro.2023.102192

Figure Lengend Snippet: Primary surface antibody staining panel: Pt25 B-cell panel

Article Snippet: Mouse monoclonal anti-Human CD3 (clone SK7) APC-Cy7 , BD Biosciences , Cat#561800; RRID: AB_396890.

Techniques: Staining

Journal: STAR Protocols

Article Title: Chimerism and phenotypic analysis of intraepithelial and lamina propria T cells isolated from human ileal biopsies after intestinal transplantation

doi: 10.1016/j.xpro.2023.102192

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-Human CD3 (clone SK7) APC-Cy7 , BD Biosciences , Cat#561800; RRID: AB_396890.

Techniques: Recombinant, Software, Flow Cytometry

Journal: STAR Protocols

Article Title: Chimerism and phenotypic analysis of intraepithelial and lamina propria T cells isolated from human ileal biopsies after intestinal transplantation

doi: 10.1016/j.xpro.2023.102192

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-Human CD3 (clone SK7) APC-Cy7 , BD Biosciences , Cat#561800; RRID: AB_396890.

Techniques: Concentration Assay, Staining

Journal: Frontiers in Immunology

Article Title: Functional changes in cytotoxic CD8+ T-cell cross-reactivity against the SARS-CoV-2 Omicron variant after mRNA vaccination

doi: 10.3389/fimmu.2022.1081047

Figure Lengend Snippet:

Article Snippet: Mouse anti-human CD3-APC-Cy7 (Clone SP34-2) , BD Biosciences , Cat# 557757, RRID : AB_396863.

Techniques: Staining, Software